Transcription.Sequences of the RNA polymerase of S. acidocaldarius were first analyzed by Zillig and colleagues (36, 46). Conservation of protein genes. 2). Specificity of protein-coding genes of the S. acidocaldarius genomea. Nucleotide sequence accession number.The annotated genome sequence has been deposited in the GenBank/EMBL sequence database under accession no. Sulfolobus acidocaldarius is an aerobic thermoacidophilic crenarchaeon which grows optimally at 80°C and pH 2 in terrestrial solfataric springs. This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. Thank you for sharing this Journal of Bacteriology article. All of these RNA genes were successfully mapped in the genome for S. acidocaldarius and are included in the Sulfolobus database. 1), and they encode a core set of proteins which can be classified into 1,391 gene families or functional groups. 3) (23). Genus: Sulfolobus. Sulfolobus acidocaldarius. The genome was assembled using the phred-phrap-consed software package (21). Class: Thermoprotei. Marker frequency measurements, using whole-genome microarrays based on the S. solfataricus genome and on a preliminary version of this S. acidocaldarius sequence, have extended this knowledge by providing evidence for three origins in both organisms (39). This element could facilitate the intercellular exchange of chromosomal DNA that is a special characteristic of S. acidocaldarius (2, 25). The A+T-rich repeat sequences of the large clusters are identical, but they show no sequence similarity to the repeats of the small clusters which are closely similar to one another and exhibit interrupted inverted repeat structures (Fig. This fragment is almost identical in sequence to the transposase gene of a single IS element which encodes both a transposase and a resolvase (Saci2022 and 2023). Arrows indicate putative replication origins which are consistent with those predicted experimentally from marker frequency analyses (39). Enter multiple addresses on separate lines or separate them with commas. Microorganisms are constantly challenged by changes of temperatures, pH, osmolarities or nutrient limitation in their direct environment. Sulfolobus acidocaldarius is an aerobic thermoacidophilic crenarchaeon which grows optimally at 80°C and pH 2 in terrestrial solfataric springs. 3). Methanobrevibacter smithii The Sulfolobus acidocaldarius have most of, if not all of, the requirements necessary to be in the domain Archaea. The research programme proposed here aims to fill this gap in our understanding by investigating the control and coordination of cell division in the model crenarchaeote Sulfolobus acidocaldarius. Blue, red, and green numbers represent genes shared with S. acidocaldarius, S. solfataricus, and S. tokodaii, respectively. Can grow heterotrophically or autotrophically. The genes for nine ribosomal proteins, L24, L5, S14, S8, L6, L18, S5, L30, and L15, have been isolated and sequenced from the spc operon in the archaeon (Crenarchaeota) Sulfolobus acidocaldarius, and the putative amino acid sequence of the proteins coded by these genes has been determined. Restriction and modification enzymes. Sulfolobusare likely to be present in most hot springs . Key to the understanding of archaeal cells is the surface layer (S-layer), which is commonly found in Archaea but whose in vivo function is unknown. Conclusion. All short open reading frames (ORFs; <120 amino acids) yielding no sequence matches in GenBank were aligned against short ORFs identified with EasyGene in the other Sulfolobus genomes. This revealed 133 short genes in total (Table 2), of which 95 were different genes, all with previously unrecognized homologs in S. solfataricus and/or S. tokodaii. However, other proteins must be responsible for sugar transport, since S. acidocaldarius can grow on both d-glucose and dextrin, as well as other sugars (24), consistent, for example, with its encoding an α-amylase (Saci1200). Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback, Print ISSN: 0021-9193; Online ISSN: 1098-5530, Sign In to Email Alerts with your Email Address.
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